Laboratory Practices:

1. All Adenoviral work must be performed at BSL-2 within a certified Biosafety cabinet. -pipetting, harvesting infected cells, opening containers
2. All vacuum lines must be fitted with a HEPA filer (e.g. Vacushield ^TM, Gelman Science)

Precautions:

1. Adenovirus is a pathogen of respiratory and gastrointestinal mucous and eye membranes
   • Does not need to be replication-competent to cause corneal-conjunctival damage.
   • Goggles should be work when working with the agent.
2. Replication-incompetent viruses may be complemented in vivo thereby becoming replication-compentent. Always treat the agent as if replication-compentent.
3. Adenovirus is very stable and can still be infective after either/CHCL₃ treatment.
4. Signs must be used to indicate locations where Adenovirus is used and stored.

Employee Exposure:

1. Eye exposure from splash/aerosols - flush a minimum 15 minutes using eye wash. Report to Occupational Health & Safety (OHS) immediately.
2. Needlestick or non-intact skin exposure (cuts). Scrub skin 20 minutes using 10% povidone iodine solution (e.g. Betadine) with copious water. Report immediately to OHS.
3. Symptomology-Acute respiratory illness; pneumonia, conjunctival infection, pink eye, corneal inflammation with possible carification.

Personal Protection:

1. Gloves (nitrile, latex, etc.).
2. Wrap-around outer clothing when introducing vector into animals or performing necropsies.
3. Goggles (non safety-glasses).
4. N-95 or HEPA respirator, to be used with concentrated titers and highly aerosolizing procedures outside of biosafety cabinet.

Decontamination of inanimate surfaces:

Most effective germicides (15 minute minimum contact time) are phenol (5%) or sodium hypochlorite (bleach diluted to 200 ppm).